Year: 2015 | Month: December | Volume 5 | Issue 4
Status of FecB Gene Mutation in Dorper Sheep from Jammu
Abstract:
The present study was carried out to explore polymorphism in exon-8 of FecB gene using PCR-RFLP technique in Dorper sheep. Animals maintained at Government Sheep Breeding Farm, Panthal, Jammu were studied. Genomic DNA was extracted from blood samples using Phenol-Chloroform-Isoamyl method. The PCR product of 140 bp was amplified using reported primers (part of exon-8 of FecB gene). PCR products was digested for the presence of FecB mutation using AvaІІ restriction enzyme. The studied samples were monomorphic and were having only one genotype (FecB++). Our study reveals that Dorper sheep population in the present study is homozygous and non-carrier of FecB mutation.
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